Genome Imaging & Editing
PrimeDesign is a flexible and comprehensive design tool for prime editing. PrimeDesign can be utilized for the installation of substitution, insertion, and deletion edits, and is generalizable for both single and combinatorial edits. Given an edit of interest, PrimeDesign identifies all possible prime editing guide RNAs (pegRNAs) and nicking guide RNAs (ngRNAs) within a specified parameter range for the optimization of prime editing.
FISH Probes Design
Other Useful Tools
NCBI gene downloader: When we are doing Genome editing or FISH probes designing, we need to download a specific region of genes from the NCBI or other database. However, it will waste your time to calculate the upstream position or the downstream position, especially you need to edit a lot of genes. With this script, you just input the location of desire gene and the base numbers of the up or downstream, then you will get the GeneBank file form NCBI.